| PHARMACOLOGICAL STUDY |
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| Year : 2014 | Volume
: 35
| Issue : 4 | Page : 447-451 |
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Use of Curcuma longa L. extract to stain various tissue samples for histological studies
Sachin Kumar1, Narendra Nath Singh1, Arun Singh1, Neelakshi Singh2, Raman Kant Sinha3
1 Department of Oral Pathology and Microbiology, Kothiwal Dental College and Research Centre, Moradabad, India
2 Department of Oral Pathology and Microbiology, Teerthankar Mahaveer University, Moradabad, Uttar Pradesh, India
3 Department of Oral Pathology and Microbiology, Sarjug Dental College, Laheriasarai, Darbhanga, Bihar, India
Correspondence Address:
Sachin Kumar
Senior Lecturer, Department of Oral Pathology and Microbiology, Kothiwal Dental College and Research Centre, Moradabad - 244 001, Uttar Pradesh
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0974-8520.159027
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Background: Curcuma longa L. is a perennial herb and a member of the Zingiberaceae (ginger) family, which is used extensively in foods as well as in Ayurvedic and Chinese systems of medicine. Current researches have focused on its antioxidant, hepatoprotective, anti-inflammatory, anticarcinogenic and antimicrobial properties. Until now, very few studies suggested its role as a histological stain. Aim: To ascertain its efficacy to be used as a counterstain after hematoxylin, to compare it's staining ability with that of routinely used eosin dye and also to ascertain its role in various collagen diseases. Materials and Methods: Turmeric rhizomes were cut into small pieces and were dried. These dried turmeric rhizomes were milled to form fine powder, which was then processed to form dye for staining tissue structures. Results: It revealed that turmeric can be used as a counterstain after hematoxylin, its staining ability was also good and comparable to that of eosin dye with a special affinity for collagen and muscle fibers. Conclusion: Turmeric dye can be used as a histological stain, which stains similar to eosin dye and its specific affinity for collagen and muscle fibers authenticates its role in the treatment of collagen and muscle disorders. |
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