Login   |  Users Online: 216 Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Search Article 
Advanced search 
   Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts

  Table of Contents  
Year : 2015  |  Volume : 36  |  Issue : 2  |  Page : 169-173  

Clinical evaluation of Cissus quadrangularis as osteogenic agent in maxillofacial fracture: A pilot study

1 Department of Oral and Maxillofacial Surgery, K. M. Shah Dental College and Hospital, Vadodara, Gujarat, India
2 Department of Dentistry, Advance Hospital, Bhuj, Gujarat, India

Date of Web Publication3-Feb-2016

Correspondence Address:
Dr. Kruti A Shah
59, Kirtikunj Society, Near Buddhdev Colony, Karelibaug, Vadodara - 390 018, Gujarat
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-8520.175542

Rights and Permissions

Introduction: Cissus quadrangularis Linn. is an indigenous medicinal plant, grown in India, which helps to increase healing process of fractured bone. Fracture of maxillofacial skeletal takes reasonably long time to heal. Many attempts have been made till today to reduce the healing period of 6–8 weeks, by means of improved surgical technology or by inhibiting the physiological mechanism of bone healing. Aim: To evaluate the effect of C. quadrangularis in healing process of maxillofacial fracture. Materials and Methods: All the patients were treated by open reduction internal fixation method and in postoperative management, antibiotics, and analgesics. Patients were divided into two groups. In Group 1, one capsule of C. quadrangularis (500 mg) thrice a day for 6 weeks was administered (n = 5), and in Group 2 (control group), no supplementary medication was administered (n = 4). Pain, swelling, fragment mobility, serum calcium, and serum phosphorus were evaluated pre- and post-operatively on day-1, -21, and -45. Results: Pain, swelling, and fragment mobility were low in Group 1 compared to Group 2. Serum calcium and serum phosphorus were also high, and healing of bone was clearly seen in Group 1 on day 21 as compared to control group. Conclusion: C. quadrangularis helps in reducing pain, swelling, and fracture mobility and accelerate the healing of fracture jaw bones.

Keywords: Asthisanghata, Cissus quadrangularis, Hadjod, osteogenic agent

How to cite this article:
Brahmkshatriya HR, Shah KA, Ananthkumar G B, Brahmkshatriya MH. Clinical evaluation of Cissus quadrangularis as osteogenic agent in maxillofacial fracture: A pilot study. AYU 2015;36:169-73

How to cite this URL:
Brahmkshatriya HR, Shah KA, Ananthkumar G B, Brahmkshatriya MH. Clinical evaluation of Cissus quadrangularis as osteogenic agent in maxillofacial fracture: A pilot study. AYU [serial online] 2015 [cited 2023 Jan 31];36:169-73. Available from: https://www.ayujournal.org/text.asp?2015/36/2/169/175542

   Introduction Top

Ayurveda, the ancient science of medicine describes different types of herbal preparations that achieve the hastening of bone healing.[1] In Ayurveda, most of the names of the plants have been given according to their medicinal values.[2] The plant is known as Harishankar or Hadjod in Hindi and Asthisanghata, Kandavalli, Vajrangi, etc., in Sanskrit. Hadjod means that which joins the bones.[2],[3]

Cissus quadrangularis Linn. (Hadjod) is an indigenous medicinal plant grown in India, Sri Lanka, and Africa. It has been prescribed in ancient Ayurveda texts by Bhava Prakasha and Chakra Dutta as a general tonic, especially for the fractured patient. Since then, it has been used as an external application and as an internal medication by bonesetters.[1],[2],[3]

C. quadrangularis is a shrub, which has slender, fleshy fibrous, smooth stem, with four winged internodes. Stem and root portion of this plant possess antioxidant and antimicrobial activity. C. quadrangularis contains high amount of anabolic steroidal substances, calcium, and phosphorus.[2],[3] The extracts from stem of this plant have been used widely for the early repair of fractures, gout, back pain, and irregular menstruation since ancient time. The steroidal substances from C. quadrangularis have shown marked influence on the rate of fracture-healing and quicker mineralization of the callus.[4]C. quadrangularis comes in the form of capsule, tablet, and syrup. The daily adult dose requires 1000–1500 mg.

The maxillofacial fractures take at least 12–16 weeks to heal. This period reduced by means of improved surgical techniques due to recent advances in internal fixation or by inhibiting the physiological mechanism of bone healing.[5]C. quadrangularis contains high amount of Vitamin C, Vitamin A, anabolic steroidal substances, and calcium. The steroidal substances from the C. quadrangularis showed marked influence on early regeneration of all connective tissue of mesenchymal origin, and thereby, improve the bone healing.[2]

The aim of the present study was to evaluate the effect of C. quadrangularis in accelerating healing of facial fracture by reducing postoperative complication such as pain, swelling, and mobility.

   Materials and Methods Top

Nine patients, 8 male and 1 female, age from 20 to 63 years, with maxillofacial fracture, reported to the Oral and Maxillofacial Department, Sangam Multispeciality Hospital, Vadodara were included in the study. Hospital ethical committee approved the study (ref no. B-27/433), and informed consent was obtained from all patients.

Inclusion criteria

Patients with mandibular and maxillary fractures.

Exclusion criteria

Patients on steroid therapy or immune-suppressants and suffering from any other chronic debilitating diseases, fracture with severe head injury, bone loss, and infected fracture were excluded.

Grouping and posology

The patients were divided into two groups, a test group and a control group. The control group consisted of four patients, in whom no supplemental drugs were administered. Five patients were included in the test group for whom 500 mg of C. quadrangularis[2] capsule (manufactured by Aeran India Private Limited, Maharashtra, India, and marketed as Capsule Union Date of MFG: August 2012) thrice a day for 6 weeks was prescribed. The capsules of Cissus were free from any pesticides, chemicals, and heavy metals. These capsules were freshly powdered; whole herb part dried to low moisture content of 6–7% and then slowly grinded with grinders at low temperature.[6]

All the patients were treated by open reduction internal fixation under general anesthesia or local anesthesia with miniplates. Immediate postoperative for 3 days same drugs such as antibiotics (combination of injection amoxicillin, with clavulanic acid combination 625 mg intravenous [IV]) and analgesics (injection Dynapar AQ 75 mg IV) were given. After that, patients were shifted to oral antibiotic, with tablet amoxicillin with clavulanic acid 625 mg and combination of tablet diclofenac sodium (50 mg) with paracetamol (500 mg) for next 5 days.

Criteria for assessment

Pain, swelling, fragment mobility, serum calcium, and serum phosphorus were recorded pre- and post-operative.

The pain was graded as mild, moderate, and severe based upon the patient's response to visual analog scale, wherein the subjective assessment of intensity or magnitude of pain felt by patient was recorded. Scale consists of a straight line of 10 cm in length, signifying 0 - no pain, and 10 - severe pain [Figure 1].
Figure 1: Visual analog scale for pain

Click here to view

Swelling was measured by the placement of thread from anterior to posterior limit of swelling. Posterior border of ramus, tragus of ear, lateral canthus of eye, corner of mouth, and alas of nose included for the measurement of swelling. Investigator checked fracture mobility manually. Preoperative serum calcium and serum phosphorus levels, as well as two-dimensional computed tomographic scan (2D-CT), were recorded. All the patients had come for follow-up and calcium and phosphorus test on day of 1st, 21st, and 45th day of postoperative days, as well as 2D-CT radiograph, had been taken on 21st and 45th days.

   Results Top

Pain and swelling gradually reduced in C. quadrangularis group and was completely absent after 15 days [Table 1] as compare to control group [Table 2]. The mobility was completely absent in Cissus group, but there was slight mobility present in the control group, but it is not so significant (P > 0.001). Serum calcium and phosphorus levels were high or at the adequate level that will help in healing, but in control group, they were at low levels. Apart from the clinical and blood investigation, osteogenesis was evaluated with 2D-CT for all patients. After treatment, there was early periosteum reaction and bridging between the fractures ends at the site of mandibular fracture on the 21st day and complete deposition of bone fragment on 45th day [Figure 2]a,[Figure 2]b,[Figure 2]c. As compare to control group, no clinical evidence of bridging between the bony fragment on the 21st postoperative day and minimum bridging at site of fracture on day of the 45th day [Figure 3]a,[Figure 3]b,[Figure 3]c.
Table 1: Analysis of clinical variables at different follow-up in Group 1 (n=5)

Click here to view
Table 2: Analysis of clinical variables at different follow-up in Group 2 (n=4)

Click here to view
Figure 2: (a) Preoperative malunited of fracture site. (b) After 21 days, bone formation in other site of computed tomography in fracture line. (c) After 45 days, even in malunited fracture well-defined bone formation in between the fracture bone

Click here to view
Figure 3: (a) Preoperative X-ray shows fracture in right maxilla. (b) After 21 days, there is no evidence of bone formation seen on the radiographs. (c) After 45 days, evidence of very little callus formation on the bone fracture site

Click here to view

   Discussion Top

Nutrition supply is an important factor for bone healing. Calcium is one of the substances, which helps in healing of bone, but only increased intake of calcium does not improve the rate of new bone formation. The ability for absorption and utilization of calcium should be increased for the hastening of healing. C. quadrangularis acts by the stimulation of metabolism and increased uptake of the minerals calcium, sulfur, and strontium by the osteoblasts in fracture healing. Certain amino acids such as lysine help in absorption of calcium. Cissus also contains Vitamin A and C that is effective in the formation of collagen.[2],[4],[7]

The pharmacological properties of C. quadrangularis are antioxidant, free radical scavenging potential, antibacterial activity, antiosteoporosis activity, antitumor activity, antiulcer activity, analgesic activity, antiobesity activity, antipyretic activity, and bone fracture healing activity.[2],[3],[8]

In clinical trials, based on radiological and clinical observations, it was found that C. quadrangularis will cause considerable reduction in the healing time of fractures.[5] Radiologically one of study suggested an early callus formation as well as clinical pain, tenderness, and swelling were significantly absent in Cissus group.[4] It was observed that C. quadrangularis neutralizes the antianabolic effect of steroids such as cortisone in healing of fractures. Inhibition of tissue regeneration and repair and retarding formation of the specific skeletal are antianabolic effects of cortisone. This will stimulate the cells of mesenchymal origin, namely the fibroblasts, the chondroblasts, and the osteoblasts by C. quadrangularis. These cells have greater impact on osteoblastic proliferation than other cellular responses.[2],[4],[9]

It causes less amount of tissue reaction in the fractured region, which leads to optimum decalcification in the early stage with minimum of callus formation. Therefore, deposition of calcium is just enough to join two broken segments of bone, and that is why its remodeling takes much less time.[10] Cissus is also shown to cause early gain in the tensile strength of fractured bones of its normal strength at the end of 6 weeks.[11]C. quadrangularis builds up the skeletal of the fractured bone, namely its mucopolysaccharides, collagen, phosphorus, calcium, and others. Mucopolysaccharides supplies raw materials for repair. Rapid the utilization of these raw materials earlier will be completion of healing process. C. quadrangularis not only causes the greater accumulation of mucopolysaccharides but also an early disappearance of mucopolysaccharides from the fractured area, associated with the earlier calcification and firm callus formation.[2],[3],[11]

In this study, radiograph of the Cissus group shows the early callus formation and complete new bone formation at the 7–8 weeks. Various studies have mentioned that C. quadrangularis reduces the usual fracture healing time from 14–16 to 8–10 weeks.[5],[8],[11] We also noted early reduction in pain and swelling with decreased mobility at fracture site, which could be attributed to increased serum calcium and serum phosphorus levels because of administration of C. quadrangularis in test (Cissus) Group.

   Conclusion Top

C. quadrangularis has drawn much attention now-a-days for its medicinal uses because of its efficacy and safety for human use. It does not produce any toxic effects when used orally and due to its various inherent pharmacognostic properties, C. quadrangularis is recommended as a supplementary drug to aid in healing of fractures.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

   References Top

Sanyal A, Ahmad A, Sastry M. Calcite growth in Cissus quadrangularis plant extract, a traditional Indian bone healing aid. Curr Sci 2005;89:1742-5.  Back to cited text no. 1
Justin SR, Baby J. Pharmacognostic and traditional properties of Cissus quadrangularis Linn – An overview. Int J Pharm Bio Sci 2011;2:131-9.  Back to cited text no. 2
Nayar M. Pharmalogical study of the stem of Cissus quadrangularis Linn. J Sci Ind Res 1959;18:253.  Back to cited text no. 3
Deka DK, Lahon LC, Saikia J, Mukit A. Effect of Cissus quandriagularis in accelerating healing process of experimentally fractured radius-ulna of dog: A preliminary study. Indian J Pharmacol 1994;26:44-5.  Back to cited text no. 4
  Medknow Journal  
Singh V, Singh N, Pal US, Dhasmana S, Mohammad S, Singh N. Clinical evaluation of Cissus quadrangularis and Moringa oleifera and osteoseal as osteogenic agents in mandibular fracture. Natl J Maxillofac Surg 2011;2:132-6.  Back to cited text no. 5
[PUBMED]  Medknow Journal  
Srivastava GM, Saurabh S, Nagori BP. Pharmacological and therapeutic activity of Cissus quadrangularis: An overview. Int J PharmTech Res 2010;2:1298-310.  Back to cited text no. 6
Kalfas IH. Principles of bone healing. Neurosurg Focus 2001;10:E1.  Back to cited text no. 7
Udupa KN, Prasad G. Biomechanical and calcium-45 studies on the effect of Cissus quadrangularis in fracture repair. Indian J Med Res 1964;52:480-7.  Back to cited text no. 8
Jain A, Dixit J, Prakash D. Modulatory effects of Cissus quadrangularis on periodontal regeneration by bovine-derived hydroxyapatite in intrabony defects: Exploratory clinical trial. J Int Acad Periodontol 2008;10:59-65.  Back to cited text no. 9
Teware K, Singh P, Mehta R.In vitro callus induction from stem explants of Cissus quadrangularis L. (Hadjod). Int J Ayurvedic Herb Med 2012;2:135-8.  Back to cited text no. 10
Mohammad S, Pal US, Pradhan R, Singh N. Herbal remedies for mandibular fracture healing. Natl J Maxillofac Surg 2014;5:35-8.  Back to cited text no. 11
[PUBMED]  Medknow Journal  


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1], [Table 2]

This article has been cited by
1 Comparison of Teriparatide and Combination of Cissus Quadrangularis and Dalbergia Sissoo on Bone Healing Against the Control Group in Maxillofacial Fractures: A Randomized Open-label Control Trial
Gigi PG, Ankita Chugh, Kirti Chaudhry, Amanjot Kaur, Pravin Kumar, Shubham Gaur, Shailendra Kumar, Surjit Singh
Craniomaxillofacial Trauma & Reconstruction. 2022; : 1943387521
[Pubmed] | [DOI]
2 Biocompatibility and antioxidant activity of a novel carrageenan based injectable hydrogel scaffold incorporated with Cissus quadrangularis: an in vitro study
Sruthi Sairaman, M. S. Nivedhitha, Deepti Shrivastava, Meshal Aber Al Onazi, Hmoud Ali Algarni, Mohammed Mustafa, Ali Robaian Alqahtani, Nouf AlQahtani, Kavalipurapu Venkata Teja, Krishnamachari Janani, Rajalakshmanan Eswaramoorthy, M. P. Sudhakar, Mohammad Khursheed Alam, Kumar Chandan Srivastava
BMC Oral Health. 2022; 22(1)
[Pubmed] | [DOI]
3 The effect of Cissus quadrangularis L. on delaying bone loss in postmenopausal women with osteopenia: A randomized placebo-controlled trial
Saksit Benjawan, Hataikarn Nimitphong, Pramote Tragulpiankit, Olarik Musigavong, Sompop Prathanturarug, Thanika Pathomwichaiwat
Phytomedicine. 2022; : 154115
[Pubmed] | [DOI]
4 Effect of Cissus quadrangularis Hydrogel on Enhancing Osseointegration of Titanium Implant to Bone: An In Vivo Study
Ramesh Chowdhary, Srinidhi Bhat
The Journal of Contemporary Dental Practice. 2022; 23(6): 582
[Pubmed] | [DOI]
5 Ethanolic extract of Cissus quadrangularis improves vasoreactivity by modulation of eNOS expression and oxidative stress in spontaneously hypertensive rats
Anees Ahmed Syed,Mohammed Shafiq,Mohammad Irshad Reza,Pragya Bharati,Athar Husain,Pragati Singh,Kashif Hanif,Jiaur R. Gayen
Clinical and Experimental Hypertension. 2021; : 1
[Pubmed] | [DOI]
6 Tailoring Scaffolds for Orthopedic Application With Anti-Microbial Properties: Current Scenario and Future Prospects
A. Preethi,Jayesh R. Bellare
Frontiers in Materials. 2021; 7
[Pubmed] | [DOI]
7 Tradition to Pathogenesis: A Novel Hypothesis for Elucidating the Pathogenesis of Diseases Based on the Traditional Use of Medicinal Plants
Kenny Kuchta, Silke Cameron
Frontiers in Pharmacology. 2021; 12
[Pubmed] | [DOI]
8 Development of Hippotion celerio (L.) (Lepidoptera: Sphingidae) on Cissus quadrangularis L. (Vitaceae) in Bangalore, India
Kallekkattil Soumya,Aswatha Krishnamoorthy
Journal of Agricultural and Urban Entomology. 2020; 36(1)
[Pubmed] | [DOI]
9 Fabrication and characterization of Spinacia oleracea extract incorporated alginate/carboxymethyl cellulose microporous scaffold for bone tissue engineering
Govindasamy Sharmila,Chandrasekaran Muthukumaran,Shanmugam Kirthika,Sundarapandian Keerthana,Narasimhan Manoj Kumar,Jeyadharmarajan Jeyanthi
International Journal of Biological Macromolecules. 2020; 156: 430
[Pubmed] | [DOI]
10 DNA Barcoding Markers and Next Generation Sequence Analysis of Rbcl in Intraspecies Genetic Variations of Three Varieties of Cissus quadrangularis L.
Goli Penchala Prasad, G. P. Pratap, Srinivasan Marimuthu, Shyam Baboo Prasad, Arun Manohar Gurav, Anupam Kumar Mangal, N. Srikanth
[Pubmed] | [DOI]
11 Use of low-intensity pulsed ultrasound therapy for the management of bilateral mandibular condyle fracture
Guruprasad Thulasi Doss,Aswath Gopalan,Elavenil Panneerselvam,Krishnakumar Raja
European Journal of Oral and Maxillofacial Surgery. 2020; 4(1)
[Pubmed] | [DOI]
12 A review on efficacy of Cissus quadrangularis in pharmacological mechanisms
Zaki Sadiya,Malathi R,Latha V,Sibi G
International Journal of Clinical Microbiology and Biochemical Technology. 2020; 3(1): 049
[Pubmed] | [DOI]
13 Orthobiologics with phytobioactive cues: A paradigm in bone regeneration
Prerna Singh,Archita Gupta,Irfan Qayoom,Sneha Singh,Ashok Kumar
Biomedicine & Pharmacotherapy. 2020; 130: 110754
[Pubmed] | [DOI]
14 Osteogenic potential of hexane and dichloromethane fraction of Cissus quadrangularis on murine preosteoblast cell line MC3T3-E1 (subclone 4)
Rabail H. Toor,Shabana Malik,Haleema Qamar,Faiza Batool,Maira Tariq,Zainab Nasir,Raazia Tassaduq,Jane B. Lian,Janet L. Stein,Gary S. Stein,Abdul R. Shakoori
Journal of Cellular Physiology. 2019;
[Pubmed] | [DOI]
15 An Assessment of the Osteogenic Potential of Cissus quadrangularis in Mandibular Fractures: A Pilot Study
Tulasi Nayak,Keerthi R
Journal of Maxillofacial and Oral Surgery. 2019;
[Pubmed] | [DOI]
16 Poly(L-lactic acid) nanofibers containing Cissus quadrangularis induced osteogenic differentiation in vitro
K Parvathi,Amit G. Krishnan,A Anitha,R. Jayakumar,Manitha B. Nair
International Journal of Biological Macromolecules. 2017;
[Pubmed] | [DOI]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Materials and Me...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded941    
    Comments [Add]    
    Cited by others 16    

Recommend this journal