|Year : 2020 | Volume
| Issue : 1 | Page : 29-35
Evaluation of nutritional value and antioxidant activity of root and leaf of Samarakhai (Byttneria herbacea Roxb.): An extra pharmacopoeial herb
Tarun Sharma1, Rabinarayan Acharya2
1 PG Department of Dravyaguna Vigyana, J S Ayurved Mahavidyalaya, Nadiad, Gujarat, India
2 Department of Dravyaguna Vigyana, Institute of Teaching and Research in Ayurveda, Jamnagar, Gujarat, India
|Date of Submission||14-Oct-2018|
|Date of Decision||28-Jan-2019|
|Date of Acceptance||09-Jan-2021|
|Date of Web Publication||30-Jul-2021|
PG Department of Dravyaguna Vigyana, J S Ayurved Mahavidyalaya, Nadiad - 387 001, Gujarat
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Samarakhai (Byttneria herbacea Roxb.), family Sterculiaceae, is one of the reputed folklore medicinal herbs, found in many parts of India. Although consumed as a vegetable since long time, its root and leaves are not yet reported for its nutritive value and antioxidant activities. Aim: The aim of this study was to evaluate nutritional value and antioxidant potential of root and leaf of B. herbacea Roxb. Materials and methods: Nutritional parameters such as carbohydrate, fat, protein, energy value, calcium, iron, zinc, manganese, phosphorus and vitamin C were evaluated. Antioxidant activity was evaluated through three test methods, i.e., 1,1-diphenyl-2-picrylhydrazyl, ferric-reducing antioxidant power (FRAP) and phosphomolybdenum assay. Results: B. herbacea roots and leaves showed the presence of total carbohydrate 46.39 g/100 g and 40.12 g/100 g, total fat 0.63 g/100 g and 1.20 g/100 g, true protein 11.46 g/100 g and 10.49 g/100 g, energy content 237.07 kcal/100 g and 213.24 kcal/100 g, iron 821.10 ppm and 889.64 ppm, zinc 9.2 ppm and 47.98 ppm, manganese 329.86 ppm and 474.59 ppm, phosphorus 0.40 ppm and 0.10 ppm and calcium 4856.84 ppm and 14964.49 ppm, respectively. The half-maximal inhibitory concentration values of the methanol extract of root, leaf and ascorbic acid were found to be 217.25 μg/ml, 131.42 μg/ml and 178.88 μg/ml, respectively. In FRAP assay, antioxidant activity of methanol extract of leaf (129.15 μM) was found to be more than root (73.13 μM). Conclusion: B. herbacea root contains high amount of true protein, carbohydrate and energy value, while micronutrients such as iron, zinc, manganese and calcium are more in its leaf. Both roots and leaves exhibited potent antioxidant activity where the leaves possess more values than the roots.
Keywords: Antioxidant, Byttneria, Gandhamardan hills, nutritive value, Samarakhadyam
|How to cite this article:|
Sharma T, Acharya R. Evaluation of nutritional value and antioxidant activity of root and leaf of Samarakhai (Byttneria herbacea Roxb.): An extra pharmacopoeial herb. AYU 2020;41:29-35
|How to cite this URL:|
Sharma T, Acharya R. Evaluation of nutritional value and antioxidant activity of root and leaf of Samarakhai (Byttneria herbacea Roxb.): An extra pharmacopoeial herb. AYU [serial online] 2020 [cited 2023 Jun 4];41:29-35. Available from: https://www.ayujournal.org/text.asp?2020/41/1/29/322830
| Introduction|| |
Importance of herbs in the management of human ailments cannot be over emphasized as approximately 30%–40% of today's conventional system been depending on the herbal source for their nutritional and curative purposes. Plants not only provide us phytochemicals of medicinal value but also provide many nutrients and minerals. Leafy vegetables are known as potential sources of minerals and vitamins. Among the different food articles, vegetables are the cheapest available sources of carbohydrates, proteins, vitamins and minerals., Minerals are inorganic substances, present in all body tissues and fluids, and their presence is necessary for the maintenance of certain physicochemical processes. Minerals are chemical constituents used by the body in many ways. Although they yield no energy, they have important roles to play in many activities in the body.
Plants, especially fruits and vegetables, are known to possess phytochemicals such as flavonoids and vitamins that exhibit significant amounts of antioxidant activity and that can be utilized to scavenge the excess free radicals from human body. Plants are good sources of natural antioxidants which provide protection against harmful free radicals and have been strongly associated with reduced risk of chronic diseases. Natural antioxidants exhibit many biologically important functions which include protection against oxidative stress and degenerative diseases and are reported to possess antibacterial, antiallergic, antiviral, anti-inflammatory, anticancer, antiaging activity and hepatoprotective properties.,
Samarakhai (Byttneria herbacea Roxb.), family Sterculiaceae, is a branched, unarmed herb with a perennial woody stock, glabrous ovate-acuminate toothed leaves, small pale purple flowers, has been reported for its traditional use as food and medicinal purpose. It is commonly found in peninsular India from Gujarat southwards to Tamil Nadu and in Odisha and Bihar.,, It is also known by Samarkhoi, Samarkhai, Sambarkai and Samar Kayee by the tribes of Odisha. The vernacular names denote its uses as a favorite odder (khai) of deer (sambar/samar). Different parts of the plant are used to treat different disease conditions such as wounds, fractures, cholera, dysentery, leukorrhea, and swelling., The paste of roots is being used topically for wound healing and orally for body pain. Leaves of this plant are used for dysentery, impaction and leukorrhea., The whole plant is used in cholera, diarrhea and gynecological disorders., Its roots and leaves are being cooked and eaten as vegetable in Odisha.
Recent literature review shows that only few works have been carried out on this herb regarding antioxidant activity. Antioxidant activities of aqueous extract from the leaves, stem, and root of B. herbacea, through three favorable in vitro test methods, including nitric oxide, catalase, and superoxide dismutase, have been reported. The results indicated that B. herbacea exhibits a good antioxidant activity. In spite of its importance as a food source, there are no published reports on the nutritional composition of herb. Hence, the present study reports the nutritional value including antioxidant potential of methanolic extract of root and leaf of B. herbacea.
| Materials and methods|| |
Collection, authentication and preservation of the sample
Samarakhai, growing in Gandhamardan hill ranges of Bargarh district of Odisha, India, was identified as B. herbacea Roxb., belonging to Sterculiaceae family, on the basis of its morphological characters, comparing with the reported characters mentioned in flora of Odisha and with the help of local taxonomist [Figure 1]. The fresh plant samples were collected in the month of September 2017 from its natural habitat. Plant specimen is authenticated by BSI Kolkata with letter no. CNH/2016/Tech. II/68. Herbarium was prepared and submitted to museum of Pharmacognosy Laboratory, I.P.G.T. and R.A., Jamnagar, vide herbarium no. Phm. 6200/16-17, for future reference [Figure 2]. The collected plant samples were shaken to remove adherent soil and dirt. The roots and leaves were separated from the stem and washed under running fresh water [Figure 3] and [Figure 4]. Then, they were shade dried, powdered, passed through mesh no. 80 and preserved in an airtight glass container.
The sample energy value was estimated (in kcal) by multiplying the percentage crude protein, crude lipid and carbohydrate by the recommended factor (2.44, 8.37 and 3.57, respectively) used in the analysis. The caloric value was determined based on the Atwater factor, total carbohydrates content were estimated by phenol-sulfuric acid method and total soluble protein content was estimated by the Folin–Lowry method. Total fat was estimated using the Soxhlet extraction method, total protein content was determined by Micro-Kjeldahl method, Vitamin C by 2,4-dinitrophenylhydrazine method and Vitamin A by official methods of food analysis. Microwave plasma-atomic emission spectrometry was used to determine iron, zinc, manganese and calcium. The vanadomolybdophosphoric acid colorimetric method was used to determine phosphorus content of acid extractions.
1,1-diphenyl-2-picrylhydrazyl radical scavenging
The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was performed by the method of Blois. Antioxidant activities of the methanolic extracts of the different samples were expressed as half-maximal inhibitory concentration (IC50), defined as the concentration of the test material required to cause a 50% decrease in initial DPPH concentration. Ascorbic acid was used as standard.
The ferric-reducing antioxidant power (FRAP) assay was assessed according to Benzie and Strain using a Hewlett-Packard 8453 diode array spectrophotometer.
The total antioxidant capacity of the methanolic extracts of root and leaf samples was measured spectrophotometrically using the phosphomolybdenum (PM) method.
| Results and Discussion|| |
The results of nutritional analysis of dried root and leaf of B. herbacea are presented in [Table 1].
|Table 1: Nutritional values of root and leaf of Byttneria herbacea Roxb.|
Click here to view
The energy value was found 237.07 kcal/100 g in root and 213.24 kcal/100 g in leaf. Lower calorific value of leaf makes it good in the diet of the obese. Carbohydrate, proteins, and fats are the main sources of energy. Fats have the greatest amount of food energy per mass 9 cal/g. Proteins and most carbohydrates have about 4 cal/g. The root was found to be rich in carbohydrate (46.39 g/100 g) and true protein content (11.46 g/100 g) which is comparatively little higher than the leaf, where carbohydrate and true protein are 40.12 g/100 g and 10.49 g/100 g, respectively. Intake of rich carbohydrate diet regularly prevents the formation of ketone bodies and also helps in breaking down of fatty acids. Food proteins have been marketed as functional food ingredients for prevention of various lifestyle diseases and improving human well-being.
Fat was found more in leaf (1.20 g/100 g) than the root (0.63 g/100 g). Lipids help the body to absorb fat-soluble vitamins such as vitamins A and E. Hence, it can be depicted that a diet including leaf of B. herbacea should be more palatable than that with root because dietary fats function to increase food palatability by absorbing and retaining flavors. A diet providing 1%–2% of its caloric energy as fat is said to be sufficient to human beings, as excess fat consumption yields to certain cardiovascular disorders such as atherosclerosis, cancer, and aging. Crude protein was found more in leaf (11.29%) than the root (8.17%). According to the Food and Nutrition Board, food plants that provide more than 12% of their calorific value of protein are a good source of protein.
Vitamin A was found only in leaf and not detected in the root of B. herbacea. Vitamin A (retinol) is an essential nutrient needed in small amounts by humans for the normal functioning of the visual system, growth and development, immune function, and reproduction. Vitamin C was found slightly more in leaf (23.35 mg/100 g) compared to root (23.21 mg/100 g). The current recommended dietary allowance for Vitamin C for adult men and women is set at 75 mg/day for women and 90 mg/day for men. Vitamin C is potentially involved in cancer and cardiovascular disease prevention.
Minerals are important in the diet because they serve as cofactors for many physiologic and metabolic functions. The biological effects of the trace elements in living system strongly depend on their concentration and thus should be carefully controlled, especially when herbs and drugs are used in human. The leaf was found to be rich in iron (889.64 ppm), zinc (47.98 ppm) and manganese (474.59 which is comparatively higher than the root where iron, zinc and manganese are 821.10 ppm, 9.2 ppm and 329.86 ppm, respectively. In cellular respiration, iron functions as essential components of enzymes involved in biological oxidation such as cytochromes c, c1 and a1. Iron is involved in synthesis and packaging of neurotransmitters and their uptake and degradation into other iron-containing proteins which may directly or indirectly alter brain function. Zinc-dependent enzymes are involved in macronutrient metabolism and cell replication. Vitamin A and E metabolism and bioavailability are dependent on zinc status. It is needed for tissue repair and wound healing, plays a vital role in protein synthesis and digestion, and is necessary for optimum insulin action as zinc is an integral constituent of insulin. Manganese is a part of enzymes involved in pyruvate metabolism, urea formation, and galactosyltransferase of connective tissue biosynthesis.
Calcium content was found more in leaf (14964.49 ppm) compared to root (4856.84 ppm). According to Mitchell and Curzon, the mean requirement of calcium is 9.8 mg/kg/day or about 640 mg (16 mmol) at a mean body weight of 65 kg. Calcium functions as a constituent of bones and teeth, regulation of nerve and muscle function. A reduced extracellular blood calcium increases the irritability of nerve tissue, and very low levels may cause spontaneous discharges of nerve impulses leading to tetany and convulsions., Phosphorus content is found more in root (0.40 ppm) in comparison to leaf (0.10 ppm). Phosphorus is located in every cell of the body and is vitally concerned with many metabolic processes, including those involving the buffers in body fluids.
1,1-diphenyl-2-picrylhydrazyl radical scavenging activity
The DPPH radical has been used widely to test the antioxidant activities of plant extracts. This method is based on the reduction of DPPH in methanol solution in the presence of a hydrogen-donating antioxidant, bringing about a color change from purple to yellow, which is measured at 517 nm. The IC50 values of the methanol extract of root, leaf, and ascorbic acid were found to be 217.25 μg/ml, 131.42 μg/ml and 178.88 μg/ml, respectively. Percentage scavenging of DPPH radical was found to rise with increasing concentration of the methanolic extract of root and leaf [Graph 1]a and [Graph 1]b. DPPH method allows testing of both lipophilic and hydrophilic compounds, in comparison to other methods that are restricted in the nature of antioxidants that they can be used to quantify. Based on these facts, DPPH assay is one of the most widely employed methods for screening antioxidant activities of plant extracts.
Ferric-reducing antioxidant power (FRAP) assay
In this assay, reduction of the ferric-TPTZ to the ferrous complex forms an intense blue color which can be measured at a wavelength of 593 nm. The intensity of the color is related to the amount of antioxidant reductants in the samples. The ferric-reducing activities of B. herbacea root and leaf are 73.130 μM and 129.15 μM, respectively. The absorbance of test drugs clearly increased, due to the formation of the Fe2+-TPTZ complex with increasing concentration [Graph 2].
FRAP is an advanced method to assess the reduced concentration of ferric ions. FRAP assay estimates the reducing potential of an antioxidant reacting with a ferric-tripyridyltriazine (Fe3+-TPTZ) complex and generating a colored ferrous tripyridyltriazine (Fe2+-TPTZ). It incorporates the simultaneous use of ferricyanide and ferric ions as chromogenic oxidants supplied more favorable redox conditions for a greater variety of antioxidants. FRAP assay possesses an immediate result of a large range of individual antioxidants in dose–response manner. FRAP presents the antioxidants in the sample as a reductant in a redox-linked colorimetric reaction.
PM assay is based on the reduction of phosphate-Mo (VI) to phosphate-Mo (V) by the sample and subsequent formation of a bluish green-colored phosphate/Mo (V) complex at acid pH. This method is routinely applied in the laboratory to evaluate the total antioxidant capacity of plant extracts. Phosphomolybdenum assay of root and leaf of B. herbacea are presented in [Table 2]. [Graph 3] represents the total antioxidant capacity which evaluates the increase in concentration of methanolic extract of root and leaf, reducing capacity of antioxidant.
FRAP and PM both methods are based on the redox antioxidant reaction which allow phyto products to act as reducing agents, hydrogen donators, singlet oxygen quenchers, and metal chelators. PM assay is a quantitative method to explore the reduction reaction rate among antioxidant, oxidant and molybdenum ligand. It forms a green PM complex without induction of free metal ions solution. Hence, it shows uniqueness among in vitro antioxidant assays.
In the present era, the use of natural antioxidants for scientific research purpose is increasing. Free radical-induced oxidation can result in cell membrane disintegration, membrane protein damage, and DNA mutation which can further proliferate many life-killing diseases such as cancer, cardiovascular diseases, and liver injury. Hence, one should rely on nature as natural products exhibit unique mechanism of action on these therapeutic problems.
B. herbacea Roxb. is one of the important and widely used medicinal herbs. Root and leaf of the plant are good sources of energy and contain an appreciable amount of nutrients and minerals and can be used as nutritious food in daily life. Its root contains high amount of true protein, carbohydrate, and energy value, while micronutrients such as iron, zinc, manganese and calcium are more in its leaf. Root and leaf of the plant are rich source of Vitamin C and can be used in Vitamin C deficiency. Results indicates that B. herbacea have great potential for use as a source of natural antioxidants. Its leaf shows better antioxidant activity than root through DPPH and FRAP assay. Further investigations may be carried out to find active component of the extract and to confirm the mechanism of action.
Authors are thankful the Director IPGT & RA for providing all facilities to carry out the research works.
Financial support and sponsorship
This study was financially supported by IPGT and RA, Gujarat Ayurved University, Jamnagar, India.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Schulz V Schulz V., Hansel R., Blumenthal M., Tyler V.E. Medicinal Plants, Phytomedicines and Phytotherapy. In: Rational Phytotherapy. Springer, Berlin, Heidelberg; 2004. p. 1-42.
Ifon ET, Basir O. Nutritive value of some Nigerian leafy vegetables. Part 3. Food Chem 1979;50B:231-5.
Naik R, Borkar SD, Acharya R. A comprehensive review on Sakavarga-group of vegetables from classical texts of Ayurveda. J Drug Res Ayurvedic Sci 2017;2:91-103.
Nagendra Prasad N, Sivamurthy GR, Aradhya SM. Ipomoea aquatica, an underutilized green leafy vegetable: A review. Int J Bot 2008;4:123-9.
Soetan KO, Olaiya CO, Oyewole OE. The importance of mineral elements for humans, domestic animals and plants: A review. Afr J Food Sci 2010;4:200-22.
Rout OP, Rabinarayan A, Mishra SK. In-vitro
antioxidant potentials in leaves of Coleus aromaticus
Benth and rhizomes of Zingiber zerumbet
(L.) SM. J Appl Pharm Sci 2011;1:194-8.
Krishnaiah D, Sarbatly R, Bon A. Phytochemical antioxidants for health and medicine-A move towards nature. Biotechnol Mol Biol Rev 2007;1:97-104.
Cook NC, Samman S. Flavonoids: Chemistry, metabolism, cardioprotective effects and dietary sources. J Nutr Biochem 1996;7:66-76.
Gulcin I, Huyut Z, Elmastas M, Aboul-Enein HY. Radical scavenging and antioxidant activity of tannic acid. Arabian J Chem 2010;3:43-53.
Anonymous. The Wealth of India. Vol. II: B. New Delhi: Council of Scientific and Industrial Research; 1988. p. 350.
Anonymous. Reviews on Indian Medicinal Plants. Vol. IV: Ba-By. New Delhi: Indian Council of Medical Research; 2004. p. 512-13.
Saxena HO, Brahmam M. The Flora of Orissa. Vol. I. Bhubaneswar: Orissa Forest Development Corporation Ltd.; 1994. p. 174-5.
Sharma T, Acharya R. Review on ethnomedicinal claims, pharmacological activity, and phytochemical constituents of Samarakhadyam (Byttneria herbacea
Roxb.) Drug Res Ayurvedic Sci 2018;3:173-80.
Dey A, Gupta B, Nath De J. Traditional phytotherapy against skin diseases and in wound healing of the tribes of Purulia district, West Bengal, India. J Med Plants Res 2012;6:4825-31.
Mallik BK, Panda T, Padhy RN. Traditional herbal practices by the ethnic people of Kalahandi District of Odisha, India. Asian Pac J Trop Biomed 2012;2(2):S988-99.
Sreeramulu N, Suthari S, Ragan A, Raju VS. Ethno-botanico-medicine for common human ailments in Nalgonda and Warangal districts of Telangana, Andhra Pradesh, India. Ann Plant Sci 2013;2:220-9.
Mairh AK, Mishra PK, Kumar J, Mairh A. Traditional botanical wisdom of Birhore district of Jharkhand. Indian J Tradi Knowl 2010;9:467-70.
Singh H, Dhole PA, Baske PK, Saravanan R. Ethnobotanical observations on Deogarh district, Odisha, India. J Econ Taxon Bot 2015;39:223-65.
Somkuwar SR, Dongre UJ, Chaudhary RR, Chaturvedi A. In-vitro
screening of an antioxidant potential of Byttneria herbacea
Roxb. Int J Curr Microbiol Appl Sci 2014;3:622-9.
FAO Corporate Document Repository. Calculation of the Energy Content of Foods Energy Conversion Factors; 2006a. Available from: http://www.fao.org/ag
. [Last accessed on 2018 Sep 18].
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956;28:350-6.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Association of Official Analytical Chemists. Official Methods of Analysis of the AOAC. 15th
ed. Washington, D.C: Association of Official Analytical Chemists; 1990. p. 375-9.
Association of Official Analytical Chemists. Official Methods of Analysis (Method 925.10): Micro-Kjeldahl Method. 16th
ed. Maryland, USA: International Press; 1998b. p. 7.
Kapur, A., Haskovic, A., s268;opra-Janicijevic, A., Klepo, L., Topcagic, A., Tahirovic, I. et al
. Spectrophotometric analysis of total ascorbic acid content in various fruits and vegetables. Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 2012;38:39-42.
Kapur, Gunniff SP, editor. Official Methods of Food Analysis. 15th
ed. Washington, D.C.: Association of Official Analytical Chemists; 1990. p. 152-64.
Hammer MR. A magnetically excited microwave plasma source for atomic emission spectroscopy with performance approaching that of the inductively coupled plasma. Spectrochim Acta Part B 2008;63:456-64.
Bickelhaupt DH, White EH. Laboratory Manual of Plant and Soil Analysis. Syracuse, New York: SUNYESF; 1982. p. 67.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-250.
Benzie IF, Strain JJ. Ferric reducing antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15-27.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.
Naik R., Acharya. Nutritional evaluation of four sources plants of jīvantī. Int J Health Allied Sci 2018;7:69-74. [Full text]
Hassan LG, Umar KJ. Nutritional value of balsam apple (Momordica balsamina
L.) leaves. Pak J Nutr 2006;5:522-29.
Shang N, Chaplot S, Wu J. Food Proteins for Health and Nutrition: Proteins in Food Processing. 2nd
ed. Woodhead Publishing Series in Food Science, Technology and Nutrition, Elsevier publication; 2018. p. 301-36.
Osborne DR, Voogt P. The Analysis of Nutrients in Foods. New York, USA: Academic Press; 1978. p. 49-51.
Lindsay RC. Flavours in Food Chemistry. New York: Marcel Dekker Inc.; 1996. p. 611-2.
Davidson S, Passmore R, Brock JF, Truswell AS. Human Nutrition and Dietetics. 6th
ed. Edinburgh: Churchill Livingstone; 1975. p. 756.
Food and Nutrition Board. Dietary Reference Intake: Elements. Institute of Medicine. Washington, D.C: National Academy of Sciences; 2001.
Food and Agriculture Organization of the United Nations, World Health Organization. Human Vitamin and Mineral Requirements, Report of a Joint FAO/WHO Expert Consultation Bangkok, Thailand. Food and Agriculture Organization of the United Nations, World Health Organization; 2001. p. 87.
I.o.M. Food and Nutrition Board Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium and Carotenoids. Washington, D.C.: National Academy Press; 2000. p. 95-185.
Grosso G, Bei R, Mistretta A, Marventano S, Calabrese G, Masuelli L, et al
. Effects of Vitamin C on health: A review of evidence. Front Biosci (Landmark Ed) 2013;18:1017-29.
Jacob RA. Trace elements. In: Tietz NW, editor. Textbook of Clinical Chemistry. Philadelphia: Saunders; 1994. p. 965-80.
Malhotra VK. Biochemistry for Students. 10th
ed. New Delhi, India: Jaypee Brothers Medical Publishers (P) Ltd; 1998.
Beard JL. Iron biology in immune function, muscle metabolism and neuronal functioning. J Nutr 2001;131:568S-79S.
Hays VW, Swenson MJ. Minerals and bones. In: Dukes' Physiology of Domestic Animals. 10th
ed. London, UK: Cornell University Press; 1985. P. 449-466.
Szabo G, Chavan S, Mandrekar P, Catalano D. Acute alcoholic consumption attenuates IL-8 and MCP-1 induction in response to ex vivo
stimulation. J Clin Immunol 1999;19:67-76.
Chandra RK. Micronutrients and immune functions: An overview. Ann N Y Acad Sci 1990;587:9-16.
Mitchell HH, Curzon EG. The Dietary Requirements of Calcium and Its Significance. Actualites Scientifique et Industrielles No. 771. Paris: Hermann; 1939. p. 36-101.
Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper's Biochemistry. 25th
ed. USA: McGraw-Hill; 2000.
Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 2004;85:633-40.
Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Anal 2002;13:8-17.
Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radic Biol Med 1996;21:895-902.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996;239:70-6.
Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y. Antioxidant activities of peel, pulp and seed fractions as determined by FRAP assay. Nutr Res 2003;23:1719-26.
Rice-Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152-9.
Phatak RS, Hendre AS. Total antioxidant capacity (TAC) of fresh leaves of Kalanchoe pinnata
. J Pharmacogn Phytochem 2014;2:32-5.
Liao K, Yin M. Individual and combined antioxidant effects of seven phenolic agents in human erythrocyte membrane ghosts and phosphatidylcholine liposome systems: Importance of the partition coefficient. J Agric Food Chem 2000;48:2266-70.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2]